3.A.1.155.1
The phage infection protein of 901 aas, PIP (Geller et al. 1993). The PIP family (3.A.1.155) includes large proteins with 1 N-terminal hydrophobic
TMS, a hydrophilic domain of variable length, and 5 C-terminal putative
TMSs. The functionally characterized protein from Lactococcus lactis is of 901 aas (Geller et al., 1993). Homologues obtained with one PSI-BLAST iteration include members of the MmpL family of the RND superfamily (e.g., a Bacillus protein, gi#89208076; 1038 aas). With poorer scores, a protein
annotated as an ABC-2-like sequence (gi#89200681; 392 aas with 1 TMS
followed by a 150 residue hydrophilic domain followed by a C-terminal 5
putative TMSs) was retrieved. Another protein annotated as ABC-2 was
smaller with 6 putative TMSs in a 2 + 3 + 1 arrangement (gi#57234453;
241 aas). The hydrophilic domain in these proteins may show sequence similarity with the large periplasmic hydrophilic domains of RND porters (2.A.6.1 - 9).
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| Accession Number: | P49022 |
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| Protein Name: | Pip |
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| Length: | 901 |
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| Molecular Weight: | 99452.00 |
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| Species: | Lactococcus lactis subsp. lactis (Streptococcus lactis) [1360] |
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| Number of TMSs: | 6 |
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| Location1 / Topology2 / Orientation3: |
Cell membrane1 / Multi-pass membrane protein2 |
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| Substrate |
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| Pfam: |
PF01061
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[1] “Cloning of a chromosomal gene required for phage infection of Lactococcus lactis subsp. lactis C2.” Geller B.L. et.al. 8366036
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1: MKSIKNTWKL FALDWKRIFK NPVATFLIVA LMIIPSLYAW FNIKALWDPY ANTSQLPIAV
61: YSADKPASFQ DKTINIGDEV LKNLKKNHQL GWKFVDSKAE LDKGVKSGKY YAGIYLPKDF
121: SKDLLSFTTG DIKKPQIVYS INEKINAIAP KITSKGASSL QSQISEEFIN TASSTLLKTF
181: NTIGYDIDKN MVSIQKVKSA ILSTNDNLGT IDKYTQQVVD LHGKMPEIKE KLAKANEFIT
241: YLPEVDSLGQ KVISLNDKMP EIDKSLSLVL TLQEKIPEIQ NAGKQISMID EDFASVESTM
301: TQGIQEAKDG LQIINQVQKS MPDIQKLGQD ADSLGTVTLD AAKKMQSALP SITNSVQITL
361: QSIQEFSKNT SSVLAVIDQA IADNQLTDEE KEQINSLITN FINNIARQRE AIQNIVDYMK
421: QVQESNGSHD LDSTIEQLSN LDSLLSGLST RMNHLNELVQ NGDVSEIRAY LSQINDVVTK
481: ISNLVDNIDV SGISKTIDTV LTSLTKTIAD AKGLLNQAQQ IDFEALLNST SQTVSNAVTI
541: LEKYQGEMPA IKQEIHDANL MLNGNMTTIV NAINEGASLY KNEFPILKTK LGTASDFFKN
601: DYAGVRKDLT DTLTMANEKM PDVESALNQA NSLIQNDWPE IKSGIQKAAT AIQKGEKEVD
661: LGEIVKLLKL DATKESDFWT QPVEVQENQV YPIANNGSAS TPFYTALCLW VGAVLLSSLA
721: TTEVHLSDKD KKRYSKREQF FARMGSFVAI GLAQALIVTL GNYFGLGVDV RDPFYSVLFA
781: LLISIAFMIM VYVLVALFGN VGKGIAIIIL VLSISGGGGN YPIQVSGKFF QAINPYLPFT
841: HAVNLLRESA GGIYWPNAWF AIIILVAVSI IFVAIGAILF PHFEDKTKKI SEIGSKSHFF
901: H