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9.B.62 The Copper Resistance (CopD) Family

The CopD family consists of many homologues with 8 putative TMSs and sizes of about 300 residues from Gram-negative and Gram-positive bacteria. Some homologues from Gram-positive bacteria are larger (Listeria monocytogenes, 541 aas; Corynebacterium efficiens, 356 aas; Streptomyces coelicolor, 680 aas). Some of these proteins are multidomain proteins with extra soluble or membrane integrated domains of unknown function.

The E. coli homologue, PcoD, present in an operon (pcoABCDRS), has been characterized from plasmid pRJ1004 (Brown et al., 1995). It is homologous to the CopD protein of Pseudomonas syringae pv. tomato. The proteins in this operon appear to catalyze copper efflux in the logarithmic growth phase but allow accumulation in stationary phase (Brown et al., 1995). Other constituents present in the outer membrane (PcoB) and in the periplasm (PcoA and C) may be involved (Lee et al., 2002; Silver and Ji, 1994). Transcription of pco/cop operons may be controlled by two-component systems such as the PcoS/PcoR sensor kinase/response regulator system. The mechanism of copper resistance is not known but may involve either (1) copper efflux, (2) copper uptake plus periplasmic copper sequestration by CopA and CopC, or (3) copper uptake by a two component CopD-CopC system coupled to an unknown resistance mechanism.

This family belongs to the: Copper Resistance (CuR) Superfamily.

References associated with 9.B.62 family:

Brown, N.L., S.R. Barrett, J. Camakaris, B.T. Lee, and D.A. Rouch. (1995). Molecular genetics and transport analysis of the copper-resistance determinant (pco) from Escherichia coli plasmid pRJ1004. Mol. Microbiol. 17: 1153-1166. 8594334
Lee, S.M., G. Grass, C. Rensing, S.R. Barrett, C.J.D. Yates, J.V. Stoyanov, and N.L. Brown. (2002). The Pco proteins are involved in periplasmic copper handling in Escherichia coli. Biochem. Biophys. Res. Commun. 295: 616-620. 12099683
Mills, S.D., C.A. Jasalavich, and D.A. Cooksey. (1993). A two-component regulatory system required for copper-inducible expression of the copper resistance operon of Pseudomonas syringae. J. Bacteriol. 175: 1656-1664. 8449873
Morosov, X., C.F. Davoudi, M. Baumgart, M. Brocker, and M. Bott. (2018). The copper-deprivation stimulon of comprises proteins for biogenesis of the actinobacterial cytochrome - supercomplex. J. Biol. Chem. 293: 15628-15640. 30154248
Rehan, M., T. Furnholm, R.H. Finethy, F. Chu, G. El-Fadly, and L.S. Tisa. (2014). Copper tolerance in Frankia sp. strain EuI1c involves surface binding and copper transport. Appl. Microbiol. Biotechnol. 98: 8005-8015. 24903815
Silver, S. and G. Ji. (1994). Newer systems for bacterial resistances to toxic heavy metals. Environ Health Perspect 102Suppl3: 107-113. 7843081
Williams, C.L., H.M. Neu, Y.A. Alamneh, R.M. Reddinger, A.C. Jacobs, S. Singh, R. Abu-Taleb, S.L.J. Michel, D.V. Zurawski, and D.S. Merrell. (2020). Characterization of Copper Resistance Reveals a Role in Virulence. Front Microbiol 11: 16. 32117089