9.B.179 The MscS/DUF475 (DUF475) Family

The DUF475 family consists of proteins primarily from Actinobacteria but possibly also Firmicutes.  They have 4 N-terminal TMSs that may be related to MARVEL domain containing proteins (TC# 1.A.64) and pantophysins (TC# 9.B.130). The hydrophilic C-terminal domain is G/Q/P-rich and redundant.  The 34 kDa antigenic protein homolog, Rv0954, is a protein of 303 aas and 4 TMSs near the N-terminus of the protein.  This is one of several dominant glycoproteins that play roles in M. tuberculosis survival, and immunogenicity. They are glycoproteins involved in antigenicity and transport. They may play a role in athogen-host interactions and drug efflux pumps, which are considered as a feasible drug targets or TB new vaccine candidates (Yari et al. 2023).

This family belongs to the Tetraspan Junctional Complex Protein or MARVEL (4JC) Superfamily.



Yari, S., P. Afrough, F. Yari, M. Ghazanfari Jajin, A. Fateh, and A. Hadizadeh Tasbiti. (2023). A potent subset of Mycobacterium tuberculosis glycoproteins as relevant candidates for vaccine and therapeutic target. Sci Rep 13: 22194.


TC#NameOrganismal TypeExample

Uncharacterized protein of 289 aas and 4 TMSs


UP of Streptomyces coelicolor


DUF475/MscS related uncharacterized protein of 272 aas and 4 N-terminal TMSs.


UP of Thermomonospora curvata


Uncharacterized protein of 336 aas and 4 TMSs


UP of Streptococcus australis


Uncharacterized protein of 300 aas and 4 N-terminal TMSs


UP of Frankia sp.


The tuberculosis potential drug target/vaccine candidate, Rv0954, DUF5336, of 303 aas with 4 TMSs near the N-terminus of the protein (Yari et al. 2023). 

Rv0954 of Mycobacterium tuberculosis


DUF5336 domain-containing protein of 387 aas and 4 TMSs.

DUF5336 protein of Tsukamurella asaccharolytica