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1.A.17.1.25
TMem16A or Anoctamin-1 (Ano1) Ca2+-activated anion (Cl-) channel of 960 aas and 10 TMSs.  Its structure has been solved by cryoEM (Paulino et al. 2017). The protein shows a similar organization to the fungal nhTMEM16, except for changes at the site of catalysis. There, the conformation of transmembrane helices, constituting a membrane-spanning furrow that provides a path for lipids in scramblases, is replaced to form an enclosed aqueous pore that is largely shielded from the membrane (Paulino et al. 2017). It thus provides a pathway for anions such as Cl-.  During activation, the binding of Ca2+ to a site located within the transmembrane domain, in the vicinity of the pore, alters the electrostatic properties of the ion conduction path and triggers a conformational rearrangement of an α-helix that comes into physical contact with the bound ligand, and thereby directly couples ligand binding and pore opening (Paulino et al. 2017).  The E143A mutant showed reduced sensitivity to Ca2+ but not to high temperatures, whereas the E705V mutant exhibited reduced sensitivity to both Ca2+ and noxious heat (Choi et al. 2018).

Accession Number:Q8BHY3
Protein Name:Anoctamin-1
Length:960
Molecular Weight:110916.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:8
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate inorgamic anions, chloride ions, Cl-

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FASTA formatted sequence
1:	MRVPEKYSTL PAEDRSVHIV NICAIEDLGY LPSEGTLLNS LSVDPDAECK YGLYFRDGKR 
61:	KVDYILVYHH KRASGSRTLA RRGLQNDMVL GTRSVRQDQP LPGKGSPVDA GSPEVPMDYH 
121:	EDDKRFRREE YEGNLLEAGL ELENDEDTKI HGVGFVKIHA PWHVLCREAE FLKLKMPTKK 
181:	VYHISETRGL LKTINSVLQK ITDPIQPKVA EHRPQTTKRL SYPFSREKQH LFDLTDRDSF 
241:	FDSKTRSTIV YEILKRTTCT KAKYSMGITS LLANGVYSAA YPLHDGDYEG DNVEFNDRKL 
301:	LYEEWASYGV FYKYQPIDLV RKYFGEKVGL YFAWLGAYTQ MLIPASIVGV IVFLYGCATV 
361:	DENIPSMEMC DQRYNITMCP LCDKTCSYWK MSSACATARA SHLFDNPATV FFSVFMALWA 
421:	ATFMEHWKRK QMRLNYRWDL TGFEEEEEAV KDHPRAEYEA RVLEKSLRKE SRNKETDKVK 
481:	LTWRDRFPAY FTNLVSIIFM IAVTFAIVLG VIIYRISTAA ALAMNSSPSV RSNIRVTVTA 
541:	TAVIINLVVI ILLDEVYGCI ARWLTKIEVP KTEKSFEERL TFKAFLLKFV NSYTPIFYVA 
601:	FFKGRFVGRP GDYVYIFRSF RMEECAPGGC LMELCIQLSI IMLGKQLIQN NLFEIGIPKM 
661:	KKFIRYLKLR RQSPSDREEY VKRKQRYEVD FNLEPFAGLT PEYMEMIIQF GFVTLFVASF 
721:	PLAPLFALLN NIIEIRLDAK KFVTELRRPV AIRAKDIGIW YNILRGVGKL AVIINAFVIS 
781:	FTSDFIPRLV YLYMYSQNGT MHGFVNHTLS SFNVSDFQNG TAPNDPLDLG YEVQICRYKD 
841:	YREPPWSEHK YDISKDFWAV LAARLAFVIV FQNLVMFMSD FVDWVIPDIP KDISQQIHKE 
901:	KVLMVELFMR EEQGKQQLLD TWMEKEKPRD VPCNNHSPTT HPEAGDGSPV PSYEYHGDAL