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1.A.29.1.3
Proton-gated urea transport channel (UreI) (pH-sensitive). Allows the transmembrane flow of urea, hydroxyurea and (at a low rate) water. KB for urea is ~150mM (Sachs et al., 2006; Scott et al., 2010). Transport kinetics and selectivity have been defined (Gray et al., 2011).  The 3-d structure reveals a hexameric protein with a channel included within the twisted 6 TMS bundle of each protomer.  It displays a two helix hairpin structure repeated three times around the central axis of the channel (Strugatsky et al. 2012). 

Accession Number:Q09068
Protein Name:UreI aka HP0071
Length:195
Molecular Weight:21706.00
Species:Helicobacter pylori (Campylobacter pylori) [210]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Urea, Hydroxyurea

Cross database links:

HEGENOM: HBG369044
DIP: DIP-3136N
RefSeq: NP_206871.1   
Entrez Gene ID: 899995   
Pfam: PF02293   
KEGG: hpy:HP0071   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0009405 P:pathogenesis
GO:0006810 P:transport

References (9)

[1] “Expression of Helicobacter pylori urease genes in Escherichia coli grown under nitrogen-limiting conditions.”  Cussac V.et.al.   1313413
[2] “Identification of the urease operon in Helicobacter pylori and its control by mRNA decay in response to pH.”  Akada J.K.et.al.   10844692
[3] “Recombination and mutation during long-term gastric colonization by Helicobacter pylori: estimates of clock rates, recombination size and minimal age.”  Falush D.et.al.   11742075
[4] “The complete genome sequence of the gastric pathogen Helicobacter pylori.”  Tomb J.-F.et.al.   9252185
[5] “The Helicobacter pylori UreI protein is not involved in urease activity but is essential for bacterial survival in vivo.”  Skouloubris S.et.al.   9712811
[6] “A H+-gated urea channel: the link between Helicobacter pylori urease and gastric colonization.”  Weeks D.L.et.al.   10642549
[7] “Sites of pH regulation of the urea channel of Helicobacter pylori.”  Weeks D.L.et.al.   11442825
[8] “The Helicobacter pylori UreI protein: role in adaptation to acidity and identification of residues essential for its activity and for acid activation.”  Bury-Mone S.et.al.   11737644
[9] “Interactions among the seven Helicobacter pylori proteins encoded by the urease gene cluster.”  Voland P.et.al.   12388207
Structure:
3ux4     

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLGLVLLYVG IVLISNGICG LTKVDPKSTA VMNFFVGGLS IICNIVVITY SALHPTAPVE 
61:	GAEDIAQVSH HLTSFYGPAT GLLFGFTYLY AAINHTFGLD WRPYSWYSLF VAINTIPAAI 
121:	LSHYSDMLDD HKVLGITEGD WWAIIWLAWG VLWLTAFIEN ILKIPLGKFT PWLAIIEGIL 
181:	TAWIPAWLLF IQHWV