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1.A.35.3.2
Cobalt/magnesium transport protein, CorA.  The structure at 2.7 Å resolution is known. The CorA monomer has a C-terminal membrane domain containing two transmembrane segments and a large N-terminal cytoplasmic soluble domain. In the membrane, CorA forms a homopentamer shaped like a funnel which binds fully hydrated Mg2+ in the periplasm (Maguire 2006). A ring of positive charges are external to the ion-conduction pathway at the cytosolic membrane interface, and highly negatively charged helices in the cytosolic domain  appear to interact with the ring of positive charge to facilitate Mg2+ entry. Mg2+ ions are present in the cytosolic domain that are well placed to control the interaction of the ring of positive charge and the negatively charged helices, and thus, control Mg2+ entry (Maguire 2006).  Gating is achieved by helical rotation upon the binding of a metal ion substrate to the regulatory binding sites. The preference for Co2+ over Mg2+ is determined by the presence of threonine side chains in the channel (Nordin et al. 2013).

Accession Number:Q9WZ31
Protein Name:Magnesium transport protein CorA
Length:351
Molecular Weight:41446.00
Species:Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [243274]
Number of TMSs:3
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Co2+, Mg2+

Cross database links:

eggNOG: COG0598
DIP: DIP-59006N
Entrez Gene ID: 897621   
Pfam: PF01544   
KEGG: tma:TM0561   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015087 F:cobalt ion transmembrane transporter activity
GO:0015095 F:magnesium ion transmembrane transporter activity

References (3)

[1] “Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima.”  Nelson K.E.et.al.   10360571
[2] “Crystal structure of the CorA Mg2+ transporter.”  Lunin V.V.et.al.   16598263
[3] “Crystal structure of a divalent metal ion transporter CorA at 2.9 Angstrom resolution.”  Eshaghi S.et.al.   16857941
Structure:
2BBH   2BBJ   2HN2   2IUB   4EEB   4EED   4I0U     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEEKRLSAKK GLPPGTLVYT GKYREDFEIE VMNYSIEEFR EFKTTDVESV LPFRDSSTPT 
61:	WINITGIHRT DVVQRVGEFF GIHPLVLEDI LNVHQRPKVE FFENYVFIVL KMFTYDKNLH 
121:	ELESEQVSLI LTKNCVLMFQ EKIGDVFDPV RERIRYNRGI IRKKRADYLL YSLIDALVDD 
181:	YFVLLEKIDD EIDVLEEEVL ERPEKETVQR THQLKRNLVE LRKTIWPLRE VLSSLYRDVP 
241:	PLIEKETVPY FRDVYDHTIQ IADTVETFRD IVSGLLDVYL SSVSNKTNEV MKVLTIIATI 
301:	FMPLTFIAGI YGMNFEYMPE LRWKWGYPVV LAVMGVIAVI MVVYFKKKKW L