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1.B.6.1.3
OmpATb (ArfA). The central domain (residues 73-220) has been reported to exhibit channel activity (Molle et al., 2006). However through structural analyses, Teriete et al. (2010) have provided evidence that it does not form a β-barrel, causing them to propose that it does not have porin activity. Its expression is dependent on small single TMS membrane proteins which are encoded in a single operon with it (Veyron-Churlet et al., 2011). The rv0899 gene is part of an operon (rv0899-rv0901) that is required for fast ammonia secretion, pH neutralization, and growth of M. tuberculosis in acidic environments, and OmpATb may allow secretion (Song et al. 2011). Homologues are widespread in bacteria with functions in nitrogen metabolism, adaptation to nutrient poor environments, and/or establishing symbiosis with host organisms (Marassi, 2011).

Accession Number:P65593
Protein Name:Uncharacterized protein Rv0899 aka MT0922
Length:326
Molecular Weight:33574.00
Species:Mycobacterium tuberculosis [1773]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate small molecules

Cross database links:

HEGENOM: HBG565454
RefSeq: NP_215414.1    NP_335355.1   
Entrez Gene ID: 885286    926237   
Pfam: PF04972    PF00691   
KEGG: mtc:MT0922    mtu:Rv0899   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane

References (2)

[1] “Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.”  Cole S.T.et.al.   9634230
[2] “Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains.”  Fleischmann R.D.et.al.   12218036
Structure:
2KGS   2KGW   2KSM     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MASKAGLGQT PATTDARRTQ KFYRGSPGRP WLIGAVVIPL LIAAIGYGAF ERPQSVTGPT 
61:	GVLPTLTPTS TRGASALSLS LLSISRSGNT VTLIGDFPDE AAKAALMTAL NGLLAPGVNV 
121:	IDQIHVDPVV RSLDFSSAEP VFTASVPIPD FGLKVERDTV TLTGTAPSSE HKDAVKRAAT 
181:	STWPDMKIVN NIEVTGQAPP GPPASGPCAD LQSAINAVTG GPIAFGNDGA SLIPADYEIL 
241:	NRVADKLKAC PDARVTINGY TDNTGSEGIN IPLSAQRAKI VADYLVARGV AGDHIATVGL 
301:	GSVNPIASNA TPEGRAKNRR VEIVVN