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1.C.12.1.7
Listeriolysin O, LLO (Viala et al., 2008). CFTR transiently increases phagosomal chloride concentrations after infection, potentiating pore formation and vacuole lysis. Thus, Listeria exploits mechanisms of cellular ion homeostasis to escape the phagosome (Radtke et al., 2011).  LLO is an example of a large beta-barrel pore that exhibits plasticity, controlled by environmental cues like pH (Podobnik et al. 2015).  Pore formation is a multistep process involving the sequential formation of arcs, slits, small rings and larger rings before formation of transmembrane pores (Mulvihill et al. 2015).  LLO promotes nanoscale membrane reorganization (Sarangi et al. 2016). It alters lysosomal integrity in epithelial cells, but not in macrophages, inducing lysosomal membrane permeabilization and release of lysosomal content (Malet et al. 2016).

Accession Number:P13128
Protein Name:TACY aka HLY aka LISA aka HLYA aka LMO0202
Length:529
Molecular Weight:58688.00
Species:Listeria monocytogenes [1639]
Location1 / Topology2 / Orientation3: Secreted1
Substrate small molecules, large molecules

Cross database links:

HEGENOM: HBG337470
RefSeq: NP_463733.1   
Entrez Gene ID: 987033   
Pfam: PF01289   
BioCyc: LMON169963:LMO0202-MONOMER   
KEGG: lmo:lmo0202   

Gene Ontology

GO:0015485 F:cholesterol binding
GO:0019835 P:cytolysis
GO:0019836 P:hemolysis by symbiont of host erythrocytes
GO:0009405 P:pathogenesis

References (7)

[1] “Nucleotide sequence of the listeriolysin gene from a Listeria monocytogenes serotype 1/2a strain.”  Domann E.et.al.   2505236
[2] “Expression in Escherichia coli and sequence analysis of the listeriolysin O determinant of Listeria monocytogenes.”  Mengaud J.et.al.   3126142
[3] “Listeria monocytogenes isolates can be classified into two major types according to the sequence of the listeriolysin gene.”  Rasmussen O.F.et.al.   1937753
[4] “Identification and characterization of nucleotide sequence differences in three virulence-associated genes of Listeria monocytogenes strains representing clinically important serotypes.”  Vines A.et.al.   9541569
[5] “Comparative genomics of Listeria species.”  Glaser P.et.al.   11679669
[6] “Identification of the structural gene encoding the SH-activated hemolysin of Listeria monocytogenes: listeriolysin O is homologous to streptolysin O and pneumolysin.”  Mengaud J.et.al.   2824384
[7] “Attenuated mutants of the intracellular bacterium Listeria monocytogenes obtained by single amino acid substitutions in listeriolysin O.”  Michel E.et.al.   1965218

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FASTA formatted sequence
1:	MKKIMLVFIT LILVSLPIAQ QTEAKDASAF NKENSISSMA PPASPPASPK TPIEKKHADE 
61:	IDKYIQGLDY NKNNVLVYHG DAVTNVPPRK GYKDGNEYIV VEKKKKSINQ NNADIQVVNA 
121:	ISSLTYPGAL VKANSELVEN QPDVLPVKRD SLTLSIDLPG MTNQDNKIVV KNATKSNVNN 
181:	AVNTLVERWN EKYAQAYPNV SAKIDYDDEM AYSESQLIAK FGTAFKAVNN SLNVNFGAIS 
241:	EGKMQEEVIS FKQIYYNVNV NEPTRPSRFF GKAVTKEQLQ ALGVNAENPP AYISSVAYGR 
301:	QVYLKLSTNS HSTKVKAAFD AAVSGKSVSG DVELTNIIKN SSFKAVIYGG SAKDEVQIID 
361:	GNLGDLRDIL KKGATFNRET PGVPIAYTTN FLKDNELAVI KNNSEYIETT SKAYTDGKIN 
421:	IDHSGGYVAQ FNISWDEVNY DPEGNEIVQH KNWSENNKSK LAHFTSSIYL PGNARNINVY 
481:	AKECTGLAWE WWRTVIDDRN LPLVKNRNIS IWGTTLYPKY SNKVDNPIE