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2.A.42.1.2
High affinity (3 μM tryptophan permease, Mtr.  Also transports indole. It functions to scavenge trace amounts of tryptophan in the medium for protein synthesis when concentrations are very low (Gu et al. 2013).

Accession Number:P0AAD2
Protein Name:Tryptophan-specific transport protein Mtr aka B3161
Length:414
Molecular Weight:44333.00
Species:Escherichia coli [83333]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate tryptophan, Indole

Cross database links:

Genevestigator: P0AAD2
EchoBASE: EB0612
EcoGene: EG10617
eggNOG: COG0814
HEGENOM: HBG482321
RefSeq: AP_003706.1    NP_417630.1   
Entrez Gene ID: 947675   
Pfam: PF03222   
BioCyc: EcoCyc:MTR-MONOMER    ECOL168927:B3161-MONOMER   
KEGG: ecj:JW3130    eco:b3161   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0006865 P:amino acid transport

References (7)

[1] “Cloning, nucleotide sequence, and characterization of mtr, the structural gene for a tryptophan-specific permease of Escherichia coli K-12.”  Heatwole V.M.et.al.   1987112
[2] “Regulation of expression of the Escherichia coli K-12 mtr gene by TyrR protein and Trp repressor.”  Sarsero J.P.et.al.   2061290
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “The tryptophan-specific permease gene, mtr, is differentially regulated by the tryptophan and tyrosine repressors in Escherichia coli K-12.”  Heatwole V.M.et.al.   1904443
[6] “Membrane topology analysis of Escherichia coli K-12 Mtr permease by alkaline phosphatase and beta-galactosidase fusions.”  Sarsero J.P.et.al.   7814318
[7] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
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  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MATLTTTQTS PSLLGGVVII GGTIIGAGMF SLPVVMSGAW FFWSMAALIF TWFCMLHSGL 
61:	MILEANLNYR IGSSFDTITK DLLGKGWNVV NGISIAFVLY ILTYAYISAS GSILHHTFAE 
121:	MSLNVPARAA GFGFALLVAF VVWLSTKAVS RMTAIVLGAK VITFFLTFGS LLGHVQPATL 
181:	FNVAESNASY APYLLMTLPF CLASFGYHGN VPSLMKYYGK DPKTIVKCLV YGTLMALALY 
241:	TIWLLATMGN IPRPEFIGIA EKGGNIDVLV QALSGVLNSR SLDLLLVVFS NFAVASSFLG 
301:	VTLGLFDYLA DLFGFDDSAV GRLKTALLTF APPVVGGLLF PNGFLYAIGY AGLAATIWAA 
361:	IVPALLARAS RKRFGSPKFR VWGGKPMIAL ILVFGVGNAL VHILSSFNLL PVYQ