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2.A.55.2.3
Macrophage divalent cation (Mn2+ > Fe2+):H+ antiporter (catalyzes divalent cation efflux and regulates cation homeostasis), NRAMP1 or Slc11a2. (Techau et al., 2007). The G212V mutation leads to microcytic anemia and liver iron overload (Bardou-Jacquet et al., 2011). Multiple targeting motifs direct NRAMP1 into lysosomes (Cheng and Wang, 2012). The structure and topology has been studied revealing a symmetric but inversely oriented arrangement (Li et al., 2012).

Accession Number:P49279
Protein Name:NRAMP1
Length:550
Molecular Weight:59872.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Mn2+, Fe2+, H+

Cross database links:

Genevestigator: P49279 P49279
eggNOG: prNOG11368 COG1914
HEGENOM: HBG312498 HOG000152203
RefSeq: NP_000569.3   
Entrez Gene ID: 6556   
Pfam: PF01566   
OMIM: 600266  gene
607948  phenotype
610446  phenotype
KEGG: hsa:6556    hsa:6556   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005770 C:late endosome
GO:0005764 C:lysosome
GO:0030670 C:phagocytic vesicle membrane
GO:0070821 C:tertiary granule membrane
GO:0005384 F:manganese ion transmembrane transporter act...
GO:0051139 F:metal ion:hydrogen antiporter activity
GO:0042803 F:protein homodimerization activity
GO:0032147 P:activation of protein kinase activity
GO:0048002 P:antigen processing and presentation of pept...
GO:0070574 P:cadmium ion transmembrane transport
GO:0006876 P:cellular cadmium ion homeostasis
GO:0006879 P:cellular iron ion homeostasis
GO:0050829 P:defense response to Gram-negative bacterium
GO:0042832 P:defense response to protozoan
GO:0070839 P:divalent metal ion export
GO:0006954 P:inflammatory response
GO:0032623 P:interleukin-2 production
GO:0032632 P:interleukin-3 production
GO:0006826 P:iron ion transport
GO:0043091 P:L-arginine import
GO:0042116 P:macrophage activation
GO:0006828 P:manganese ion transport
GO:0045342 P:MHC class II biosynthetic process
GO:0048255 P:mRNA stabilization
GO:0060586 P:multicellular organismal iron ion homeostasis
GO:0001818 P:negative regulation of cytokine production
GO:0015707 P:nitrite transport
GO:0006909 P:phagocytosis
GO:0002606 P:positive regulation of dendritic cell antig...
GO:0032729 P:positive regulation of interferon-gamma pro...
GO:0050766 P:positive regulation of phagocytosis
GO:0002827 P:positive regulation of T-helper 1 type immu...
GO:0045944 P:positive regulation of transcription from R...
GO:0000060 P:protein import into nucleus, translocation
GO:0045730 P:respiratory burst
GO:0034341 P:response to interferon-gamma
GO:0032496 P:response to lipopolysaccharide
GO:0002369 P:T cell cytokine production
GO:0002309 P:T cell proliferation during immune response
GO:0007035 P:vacuolar acidification
GO:0042060 P:wound healing
GO:0009279 C:cell outer membrane
GO:0031902 C:late endosome membrane
GO:0005384 F:manganese ion transmembrane transporter activity
GO:0048002 P:antigen processing and presentation of peptide antigen
GO:0002606 P:positive regulation of dendritic cell antigen processing and presentation
GO:0032729 P:positive regulation of interferon-gamma production
GO:0002827 P:positive regulation of T-helper 1 type immune response
GO:0045944 P:positive regulation of transcription from RNA polymerase II promoter
GO:0002309 P:T cell proliferation involved in immune response

References (18)

[1] “Human natural resistance-associated macrophage protein: cDNA cloning, chromosomal mapping, genomic organization, and tissue-specific expression.”  Cellier M.et.al.   7964458
[2] “Identification of natural resistance-associated macrophage protein in peripheral blood lymphocytes.”  Kishi F.et.al.   8537108
[3] “Complete nucleotide sequence and genomic structure of the human NRAMP1 gene region on chromosome region 2q35.”  Marquet S.et.al.   10967134
[4] “Isolation and characterization of human Nramp cDNA.”  Kishi F.et.al.   7980580
[5] “Genomic organization and sequence of the human NRAMP gene: identification and mapping of a promoter region polymorphism.”  Blackwell J.M.et.al.   8529098
[6] “Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.”  Yu L.-R.et.al.   17924679
[7] “Identification of polymorphisms and sequence variants in the human homologue of the mouse natural resistance-associated macrophage protein gene.”  Liu J.et.al.   7717395
[8] “Alleles of the NRAMP1 gene are risk factors for pediatric tuberculosis disease.”  Malik S.et.al.   16103355
[9] “Susceptibility to Buruli ulcer is associated with the SLC11A1 (NRAMP1) D543N polymorphism.”  Stienstra Y.et.al.   16395392
[10] “Human natural resistance-associated macrophage protein: cDNA cloning, chromosomal mapping, genomic organization, and tissue-specific expression.”  Cellier M.et.al.   7964458
[11] “Identification of natural resistance-associated macrophage protein in peripheral blood lymphocytes.”  Kishi F.et.al.   8537108
[12] “Complete nucleotide sequence and genomic structure of the human NRAMP1 gene region on chromosome region 2q35.”  Marquet S.et.al.   10967134
[13] “Isolation and characterization of human Nramp cDNA.”  Kishi F.et.al.   7980580
[14] “Genomic organization and sequence of the human NRAMP gene: identification and mapping of a promoter region polymorphism.”  Blackwell J.M.et.al.   8529098
[15] “Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.”  Yu L.-R.et.al.   17924679
[16] “Identification of polymorphisms and sequence variants in the human homologue of the mouse natural resistance-associated macrophage protein gene.”  Liu J.et.al.   7717395
[17] “Alleles of the NRAMP1 gene are risk factors for pediatric tuberculosis disease.”  Malik S.et.al.   16103355
[18] “Susceptibility to Buruli ulcer is associated with the SLC11A1 (NRAMP1) D543N polymorphism.”  Stienstra Y.et.al.   16395392

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FASTA formatted sequence
1:	MTGDKGPQRL SGSSYGSISS PTSPTSPGPQ QAPPRETYLS EKIPIPDTKP GTFSLRKLWA 
61:	FTGPGFLMSI AFLDPGNIES DLQAGAVAGF KLLWVLLWAT VLGLLCQRLA ARLGVVTGKD 
121:	LGEVCHLYYP KVPRTVLWLT IELAIVGSDM QEVIGTAIAF NLLSAGRIPL WGGVLITIVD 
181:	TFFFLFLDNY GLRKLEAFFG LLITIMALTF GYEYVVARPE QGALLRGLFL PSCPGCGHPE 
241:	LLQAVGIVGA IIMPHNIYLH SALVKSREID RARRADIREA NMYFLIEATI ALSVSFIINL 
301:	FVMAVFGQAF YQKTNQAAFN ICANSSLHDY AKIFPMNNAT VAVDIYQGGV ILGCLFGPAA 
361:	LYIWAIGLLA AGQSSTMTGT YAGQFVMEGF LRLRWSRFAR VLLTRSCAIL PTVLVAVFRD 
421:	LRDLSGLNDL LNVLQSLLLP FAVLPILTFT SMPTLMQEFA NGLLNKVVTS SIMVLVCAIN 
481:	LYFVVSYLPS LPHPAYFGLA ALLAAAYLGL STYLVWTCCL AHGATFLAHS SHHHFLYGLL 
541:	EEDQKGETSG