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2.A.75.1.2
L-arginine exporter (ArgO or YggA) (Nandineni and Gowrishankar 2004).  ArgP-ArgO of E. coli exhibits transcriptional cross activity with the corresponding pair, LysG-LysE of Corynebacterium glutamicum (Marbaniang and Gowrishankar 2012).  ArgO when overproducted confers sensitivity to the toxic arginine analogue, canavanine and catalyzes export of L-lysine (Pathania and Sardesai 2015). ArgO assumes an Nin-Cout configuration, potentially forming a five-transmembrane helix bundle flanked by a cytoplasmic N-terminal domain (NTD) comprising roughly its first 38 to 43 amino acyl residues and a short periplasmic C-terminal region (CTR). Mutagenesis studies indicate that the CTR, but not the NTD, is dispensable for ArgO function in vivo, and that a pair of conserved aspartate residues, located near the opposing edges of the cytoplasmic membrane, may play a pivotal role in facilitating transmembrane Arg flux (Pathania et al. 2016). ArgO possesses a membrane topology that is distinct from that reported for LysE, with substantial variation in the topological arrangement of the proximal one-third portions of the two exporters. The Arg-translocating conduit is formed by a monomer of ArgO (Pathania et al. 2016).

Accession Number:P11667
Protein Name:ArgO (YggA)
Length:211
Molecular Weight:23176.00
Species:Escherichia coli [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate lysine, Histidine, Arginine

Cross database links:

Genevestigator: P11667
EchoBASE: EB1148
EcoGene: EG11159
eggNOG: COG1279
HEGENOM: HBG683136
RefSeq: AP_003481.1    NP_417398.1   
Entrez Gene ID: 947418   
Pfam: PF01810   
BioCyc: EcoCyc:YGGA-MONOMER    ECOL168927:B2923-MONOMER   
KEGG: ecj:JW2890    eco:b2923   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015181 F:arginine transmembrane transporter activity
GO:0006865 P:amino acid transport

References (5)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli.”  Alefounder P.R.et.al.   2546007
[4] “Evidence for an arginine exporter encoded by yggA (argO) that is regulated by the LysR-type transcriptional regulator ArgP in Escherichia coli.”  Nandineni M.R.et.al.   15150242
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MFSYYFQGLA LGAAMILPLG PQNAFVMNQG IRRQYHIMIA LLCAISDLVL ICAGIFGGSA 
61:	LLMQSPWLLA LVTWGGVAFL LWYGFGAFKT AMSSNIELAS AEVMKQGRWK IIATMLAVTW 
121:	LNPHVYLDTF VVLGSLGGQL DVEPKRWFAL GTISASFLWF FGLALLAAWL APRLRTAKAQ 
181:	RIINLVVGCV MWFIALQLAR DGIAHAQALF S