| |
|---|---|
| Accession Number: | O14745 |
| Protein Name: | EBP50 aka NHERF-1 |
| Length: | 358 |
| Molecular Weight: | 38868.00 |
| Species: | Homo sapiens (Human) [9606] |
| Location1 / Topology2 / Orientation3: | Endomembrane system1 / Peripheral membrane protein2 |
| Substrate | |
Cross database links:
| DIP: | DIP-29092N DIP-29092N |
|---|---|
| RefSeq: | NP_004243.1 |
| Entrez Gene ID: | 9368 |
| Pfam: | PF09007 PF00595 |
| OMIM: |
604990 gene 612287 phenotype |
| KEGG: | hsa:9368 hsa:9368 |
Gene Ontology
GO:0015629
C:actin cytoskeleton
GO:0012505
C:endomembrane system
GO:0030175
C:filopodium
GO:0043231
C:intracellular membrane-bounded organelle
GO:0005902
C:microvillus
GO:0001726
C:ruffle
GO:0031698
F:beta-2 adrenergic receptor binding
GO:0030165
F:PDZ domain binding
GO:0006461
P:protein complex assembly
GO:0032415
P:regulation of sodium:hydrogen antiporter ac...
GO:0016055
P:Wnt receptor signaling pathway
GO:0016324
C:apical plasma membrane
GO:0031526
C:brush border membrane
GO:0005813
C:centrosome
GO:0031528
C:microvillus membrane
GO:0017081
F:chloride channel regulator activity
GO:0030036
P:actin cytoskeleton organization
GO:0007191
P:adenylate cyclase-activating dopamine receptor signaling pathway
GO:0060158
P:phospholipase C-activating dopamine receptor signaling pathway
GO:0006915
P:apoptotic process
GO:0032782
P:bile acid secretion
GO:0030643
P:cellular phosphate ion homeostasis
GO:0034613
P:cellular protein localization
GO:0034635
P:glutathione transport
GO:0030033
P:microvillus assembly
GO:0030336
P:negative regulation of cell migration
GO:0008285
P:negative regulation of cell proliferation
GO:0070373
P:negative regulation of ERK1 and ERK2 cascade
GO:0014067
P:negative regulation of phosphatidylinositol 3-kinase cascade
GO:0010642
P:negative regulation of platelet-derived growth factor receptor signaling pathway
GO:0051898
P:negative regulation of protein kinase B signaling cascade
GO:0010766
P:negative regulation of sodium ion transport
GO:0032416
P:negative regulation of sodium:hydrogen antiporter activity
GO:0044062
P:regulation of excretion
GO:0045859
P:regulation of protein kinase activity
GO:0032415
P:regulation of sodium:hydrogen antiporter activity
GO:0097291
P:renal phosphate ion absorption
GO:0003096
P:renal sodium ion transport
| |
References (62)[1] “Identification of EBP50: a PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family.” Reczek D.et.al. 9314537 [2] “NHE-RF, a regulatory cofactor for Na(+)-H+ exchange, is a common interactor for merlin and ERM (MERM) proteins.” Murthy A.et.al. 9430655 [3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).” The MGC Project Teamet.al. 15489334 [4] “Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.” Gevaert K.et.al. 12665801 [5] “cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein.” Yun C.H.C.et.al. 9096337 [6] “A kinase-regulated PDZ-domain interaction controls endocytic sorting of the beta2-adrenergic receptor.” Cao T.T.et.al. 10499588 [7] “Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton.” Brdickova N.et.al. 11684085 [8] “Identification of EPI64, a TBC/rabGAP domain-containing microvillar protein that binds to the first PDZ domain of EBP50 and E3KARP.” Reczek D.et.al. 11285285 [9] “Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor.” Liedtke C.M.et.al. 11956211 [10] “Ezrin-radixin-moesin-binding phosphoprotein-50/Na+/H+ exchanger regulatory factor (EBP50/NHERF) blocks U50,488H-induced down-regulation of the human kappa opioid receptor by enhancing its recycling rate.” Li J.-G.et.al. 12004055 [11] “Epithelial inducible nitric-oxide synthase is an apical EBP50-binding protein that directs vectorial nitric oxide output.” Glynne P.A.et.al. 12080081 [12] “Regulation of GTP-binding protein alpha q (Galpha q) signaling by the ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50).” Rochdi M.D.et.al. 12193606 [13] “The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3-cotransport isoform 3.” Park M.et.al. 12403779 [14] “The PDZ-interacting domain of TRPC4 controls its localization and surface expression in HEK293 cells.” Mery L.et.al. 12154080 [15] “Estrogen receptor inducibility of the human Na+/H+ exchanger regulatory factor/ezrin-radixin-moesin binding protein 50 (NHE-RF/EBP50) gene involving multiple half-estrogen response elements.” Ediger T.R.et.al. 12145337 [16] “The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ motifs involved in their interaction.” Pushkin A.et.al. 12444018 [17] “EBP50, a beta-catenin-associating protein, enhances Wnt signaling and is over-expressed in hepatocellular carcinoma.” Shibata T.et.al. 12830000 [18] “The PDZ-binding chloride channel ClC-3B localizes to the Golgi and associates with cystic fibrosis transmembrane conductance regulator-interacting PDZ proteins.” Gentzsch M.et.al. 12471024 [19] “Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.” Olsen J.V.et.al. 17081983 [20] “Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.” Yu L.-R.et.al. 17924679 [21] “Phosphoproteome of resting human platelets.” Zahedi R.P.et.al. 18088087 [22] “Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.” Cantin G.T.et.al. 18220336 [23] “A quantitative atlas of mitotic phosphorylation.” Dephoure N.et.al. 18669648 [24] “MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells.” Arnaud C.et.al. 19555689 [25] “Structural basis of the Na+/H+ exchanger regulatory factor PDZ1 interaction with the carboxyl-terminal region of the cystic fibrosis transmembrane conductance regulator.” Karthikeyan S.et.al. 11304524 [26] “Crystal structure of the PDZ1 domain of human Na(+)/H(+) exchanger regulatory factor provides insights into the mechanism of carboxyl-terminal leucine recognition by class I PDZ domains.” Karthikeyan S.et.al. 11352585 [27] “Structural determinants of the Na+/H+ exchanger regulatory factor interaction with the beta 2 adrenergic and platelet-derived growth factor receptors.” Karthikeyan S.et.al. 11882663 [28] “The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain.” Finnerty C.M.et.al. 15020681 [29] “Structural basis for NHERF recognition by ERM proteins.” Terawaki S.et.al. 16615918 [30] “NHERF1 mutations and responsiveness of renal parathyroid hormone.” Karim Z.et.al. 18784102 [31] “Identification of EBP50: a PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family.” Reczek D.et.al. 9314537 [32] “NHE-RF, a regulatory cofactor for Na(+)-H+ exchange, is a common interactor for merlin and ERM (MERM) proteins.” Murthy A.et.al. 9430655 [33] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).” The MGC Project Teamet.al. 15489334 [34] “Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.” Gevaert K.et.al. 12665801 [35] “cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein.” Yun C.H.C.et.al. 9096337 [36] “A kinase-regulated PDZ-domain interaction controls endocytic sorting of the beta2-adrenergic receptor.” Cao T.T.et.al. 10499588 [37] “Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton.” Brdickova N.et.al. 11684085 [38] “Identification of EPI64, a TBC/rabGAP domain-containing microvillar protein that binds to the first PDZ domain of EBP50 and E3KARP.” Reczek D.et.al. 11285285 [39] “Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor.” Liedtke C.M.et.al. 11956211 [40] “Ezrin-radixin-moesin-binding phosphoprotein-50/Na+/H+ exchanger regulatory factor (EBP50/NHERF) blocks U50,488H-induced down-regulation of the human kappa opioid receptor by enhancing its recycling rate.” Li J.-G.et.al. 12004055 [41] “Epithelial inducible nitric-oxide synthase is an apical EBP50-binding protein that directs vectorial nitric oxide output.” Glynne P.A.et.al. 12080081 [42] “Regulation of GTP-binding protein alpha q (Galpha q) signaling by the ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50).” Rochdi M.D.et.al. 12193606 [43] “The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3-cotransport isoform 3.” Park M.et.al. 12403779 [44] “The PDZ-interacting domain of TRPC4 controls its localization and surface expression in HEK293 cells.” Mery L.et.al. 12154080 [45] “Estrogen receptor inducibility of the human Na+/H+ exchanger regulatory factor/ezrin-radixin-moesin binding protein 50 (NHE-RF/EBP50) gene involving multiple half-estrogen response elements.” Ediger T.R.et.al. 12145337 [46] “The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ motifs involved in their interaction.” Pushkin A.et.al. 12444018 [47] “EBP50, a beta-catenin-associating protein, enhances Wnt signaling and is over-expressed in hepatocellular carcinoma.” Shibata T.et.al. 12830000 [48] “The PDZ-binding chloride channel ClC-3B localizes to the Golgi and associates with cystic fibrosis transmembrane conductance regulator-interacting PDZ proteins.” Gentzsch M.et.al. 12471024 [49] “Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.” Olsen J.V.et.al. 17081983 [50] “Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.” Yu L.-R.et.al. 17924679 [51] “Phosphoproteome of resting human platelets.” Zahedi R.P.et.al. 18088087 [52] “Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.” Cantin G.T.et.al. 18220336 [53] “A quantitative atlas of mitotic phosphorylation.” Dephoure N.et.al. 18669648 [54] “MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells.” Arnaud C.et.al. 19555689 [55] “Initial characterization of the human central proteome.” Burkard T.R.et.al. 21269460 [56] “A new human NHERF1 mutation decreases renal phosphate transporter NPT2a expression by a PTH-independent mechanism.” Courbebaisse M.et.al. 22506049 [57] “Structural basis of the Na+/H+ exchanger regulatory factor PDZ1 interaction with the carboxyl-terminal region of the cystic fibrosis transmembrane conductance regulator.” Karthikeyan S.et.al. 11304524 [58] “Crystal structure of the PDZ1 domain of human Na(+)/H(+) exchanger regulatory factor provides insights into the mechanism of carboxyl-terminal leucine recognition by class I PDZ domains.” Karthikeyan S.et.al. 11352585 [59] “Structural determinants of the Na+/H+ exchanger regulatory factor interaction with the beta 2 adrenergic and platelet-derived growth factor receptors.” Karthikeyan S.et.al. 11882663 [60] “The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain.” Finnerty C.M.et.al. 15020681 | |
| Structure: | |
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| FASTA formatted sequence |
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1: MSADAAAGAP LPRLCCLEKG PNGYGFHLHG EKGKLGQYIR LVEPGSPAEK AGLLAGDRLV 61: EVNGENVEKE THQQVVSRIR AALNAVRLLV VDPETDEQLQ KLGVQVREEL LRAQEAPGQA 121: EPPAAAEVQG AGNENEPREA DKSHPEQREL RPRLCTMKKG PSGYGFNLHS DKSKPGQFIR 181: SVDPDSPAEA SGLRAQDRIV EVNGVCMEGK QHGDVVSAIR AGGDETKLLV VDRETDEFFK 241: KCRVIPSQEH LNGPLPVPFT NGEIQKENSR EALAEAALES PRPALVRSAS SDTSEELNSQ 301: DSPPKQDSTA PSSTSSSDPI LDFNISLAMA KERAHQKRSS KRAPQMDWSK KNELFSNL