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9.A.52.1.1
Microcin J25 (MccJ25) cyclic 21 aa peptide, derived from a precursor of 58 aas. The Ile13 residue of microcin J25 is essential for recognition by the receptor FhuA, but not by the inner membrane transporter SbmA (Socias et al., 2009). It inhibits transcription by binding deep within RNAP secondary channel, where it sterically blocks the folding of the trigger loop, which is essential for efficient catalysis (Mukhopadhyay et al. 2004; Braffman et al. 2019). It also acts on the cytoplasmic membrane of Salmonella newport, producing alteration of membrane permeability and subsequent gradient dissipation, which inhibits several processes essential for cell viability, such as oxygen consumption (Rintoul et al. 2001).

Accession Number:Q9X2V7
Protein Name:Microcin J25
Length:58
Molecular Weight:6200.00
Species:Escherichia coli [562]
Location1 / Topology2 / Orientation3: Secreted1
Substrate ion, cation

Cross database links:

Gene Ontology

GO:0005576 C:extracellular region
GO:0005102 F:receptor binding
GO:0019835 P:cytolysis
GO:0042742 P:defense response to bacterium

References (9)

[1] “Sequence analysis of the four plasmid genes required to produce the circular peptide antibiotic microcin J25.”  Solbiati J.O.et.al.   10198038
[2] “The cyclic structure of microcin J25, a 21-residue peptide antibiotic from Escherichia coli.”  Blond A.et.al.   10092860
[3] “Microcin 25, a novel antimicrobial peptide produced by Escherichia coli.”  Salomon R.A.et.al.   1429464
[4] “The antibacterial action of microcin J25: evidence for disruption of cytoplasmic membrane energization in Salmonella newport.”  Rintoul M.R.et.al.   11731133
[5] “Escherichia coli RNA polymerase is the target of the cyclopeptide antibiotic microcin J25.”  Delgado M.A.et.al.   11443089
[6] “Mutations of bacterial RNA polymerase leading to resistance to microcin J25.”  Yuzenkova J.et.al.   12401787
[7] “Structure of antibacterial peptide microcin J25: a 21-residue lariat protoknot.”  Bayro M.J.et.al.   14531661
[8] “Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone.”  Rosengren K.J.et.al.   14531690
[9] “Structure of microcin J25, a peptide inhibitor of bacterial RNA polymerase, is a lassoed tail.”  Wilson K.-A.et.al.   14531691
Structure:
1PP5   1Q71   1S7P   2MMT   2MMW   4CU4   5T56   6N60     

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FASTA formatted sequence 
1:	MIKHFHFNKL SSGKKNNVPS PAKGVIQIKK SASQLTKGGA GHVPEYFVGI GTPISFYG