9.B.198.2.1
Membrane-anchored lipid-binding protein YSP2 or LAM2 (also called LTC4) of 1438 aas and 1 or more TMSs. It is involved in induction of programmed cell death in response to reactive oxygen species (ROS) (Sokolov et al. 2006) and may be involved in sterol transfer between intracellular membranes (Gatta et al. 2015). Ergosterol, the major sterol in fungal membranes, is critical for
defining membrane fluidity and regulating cellular processes. Ergosterol synthesis is well defined in model yeast. Choy et al. 2023 identified Ysp2 as a retrograde sterol transporter,, in the
opportunistic fungal pathogen Cryptococcus neoformans. They found
that the lack of Ysp2 under host-mimicking conditions leads to abnormal
accumulation of ergosterol at the plasma membrane, invagination of the
plasma membrane, and malformation of the cell wall, which can be
functionally rescued by inhibiting ergosterol synthesis with the
antifungal drug fluconazole. They also observed that cells lacking Ysp2
mislocalize the cell surface protein Pma1 and have thinner and more
permeable capsules (Choy et al. 2023).
|
| Accession Number: | Q06681 |
|---|
| Protein Name: | Membrane-anchored lipid-binding protein YSP2 |
|---|
| Length: | 1438 |
|---|
| Molecular Weight: | 160578.00 |
|---|
| Species: | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [559292] |
|---|
| Number of TMSs: | 4 |
|---|
| Location1 / Topology2 / Orientation3: |
Mitochondrion membrane1 / Single-pass membrane protein2 |
|---|
| Substrate |
|
|---|
1: MRDEATRKKR SFSDGHFFKK LKLMSRKKQP VMERSKTTRT RKESTNSAAK SSLSLRRANN
61: GRKTIAKRRV LTDIGSTNEG VAGNSGSNSP AQYSHTPHFS DSIPPLPLEL PDIVSIRSSR
121: SHISNKSNKN KHGIDLTFIP RRSLQNSKAG LKKPNTSPQG YFNIPVTIDR ASEKVKHTDT
181: KNTFNSSSSE NERPVLSILQ KDDSQSSSHP AIDSMSAPNN INNNNDIENS SNSLFDTILS
241: IAHSAISHVP KISALNTEIQ REFSHSGESH TGSTRHPYFH IHHAQQQHPL SQQQGPLPVS
301: ENANQNPNDT VLIHSPSANT AHRSSSFLRH LDYLLSPTSG PASDKHTQVE EGDDEEELSP
361: LSKAFLSPST QLVPTNTSTT PLSGSLTPNN RNVNANSNSE TENDNDRDDR SNVGKVKFQP
421: LKVHEPAIST FGKGNLTLEA VAGSSDIDNT TIDLDENNTN NNPNASSTNL SHISKSNVNN
481: NLGPKELNTS YRNSTYIDMA RFENSQSNLS SHRARSKTLP ANKALENAVG DEGNSKRNSR
541: YSSYSNDMAF DDADERKFRS MSKKFLNRRS FSPSNLGNKV IPGINLRNSF NKNRNSSSDF
601: FSTNQGQQMP RTSTAGSGNI HAIMGLDSGN DDFKLEGIEY ASEKKNSEFH TLFKDCDINP
661: NEKLIVDHSC ALSRDILLQG RMYISDAHIG FFSNILGWVS TVFIPFKEIV QIEKKTTAGI
721: FPNGIVIDTL HTKYIFASFM SRDATFDLIT DVWNQIILGK KYRNGFGNND DGTISDSSSA
781: FFDDSDDNDD DGDLDDDDPD INSTDMTSSD DIDADVFNES NDLGKNQKST NYLLGPNKHS
841: PTTADFKPSN NDHLVIEANI NAPLGKVVNL LYGEDVSYYE RILKAQKNFE ISPIPNNFLT
901: KKIRDYAYTK PLSGSIGPSK TKCLITDTLE HYDLEDYVKV LSITKNPDVP SGNIFSVKTV
961: FLFSWDKNNS TKLTVYNSVD WTGKSWIKSM IEKGTFDGVA DTTKIMISEI KKILSDEDSN
1021: INSKHQASNN ESEEEIINLP TIGPPVHDPT EPDFQKGKDD TVIDEKINIP VPLGTVFSLL
1081: YGDDTSYIKK IIENQNNFNV CDIPKFVNNA REITYTKKLN NSFGPKQTKC IVTETIEHMD
1141: LNSFFMVKQI VRSPDVPYGS SFSVHTRFFY SWGDHNTTNM KVVTNVVWTG KSMLKGTIEK
1201: GSIDGQRSST KQLVDDLKKI ISNASSTKKK SRRRGKTVNK RKSSPSTIKN EKNEENFEDT
1261: STKNSFFSAF SMLQQVNITS VQGIMTIISF FICLIFFFRL LFHSKNTSNI QIITPGTILI
1321: NGNEYNYVPN FKTLYHVYED NIIKDARRKD SNKNNIVTDT EGLIWDWLID RGNGTVQNSV
1381: LSNHIKESNN KKVKLVNGVS DHKIQQLVES IKITELQLQE MKELLAQTDN TSATNQLL