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2.A.15.1.10
Glycine betaine transporter, BetP. The x-ray structure is known (3PO3; 2WIT; Ressl et al., 2009). Regulatory crosstalk in the trimeric BetP has been reported (Gärtner et al., 2011). An extracellular K+ -dependent interaction site modulates betaine-binding (Ge et al., 2011). The porter is trimeric and exhibits structural asymmetry (Tsai et al., 2011). The C-terminal domain is involved in osmosensing and is trimeric like wild-type BetP.  The two Na+ binding sites are between TMSs 1 and 8 in the first and second 5 TMS repeats, and between the equivalent TMSs 6 and 3 in the second and first repeats, respectively (Khafizov et al. 2012). interdependent binding of betaine and two sodium ions is observed during the coupling process. All three sites undergo progressive reshaping and dehydration during the alternating-access cycle, with the most optimal coordination of all substrates found in the closed state (Perez et al. 2014). BetP is active and regulated only when negatively charged lipids such as phosphatidyl glycerol are present, and the mechanism has been discussed (Güler et al. 2016).  The K+-sensing C-terminal domain results in K+-dependent cooperative betaine-binding (Ge et al. 2011).

Accession Number:P54582
Protein Name:Glycine betaine transporter BetP
Length:595
Molecular Weight:64209.00
Species:Corynebacterium glutamicum [1718]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Glycine betaine, K+

Cross database links:

HEGENOM: HBG682943
Entrez Gene ID: 1018885    3342778   
Pfam: PF02028   
KEGG: cgb:cg1016    cgl:NCgl0856   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity

References (1)

[1] “The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.”  Kalinowski J.et.al.   12948626
Structure:
2WIT   3P03   4AIN   4C7R   4DOJ   4LLH     

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FASTA formatted sequence
1:	MTTSDPNPKP IVEDAQPEQI TATEELAGLL ENPTNLEGKL ADAEEEIILE GEDTQASLNW 
61:	SVIVPALVIV LATVVWGIGF KDSFTNFASS ALSAVVDNLG WAFILFGTVF VFFIVVIAAS 
121:	KFGTIRLGRI DEAPEFRTVS WISMMFAAGM GIGLMFYGTT EPLTFYRNGV PGHDEHNVGV 
181:	AMSTTMFHWT LHPWAIYAIV GLAIAYSTFR VGRKQLLSSA FVPLIGEKGA EGWLGKLIDI 
241:	LAIIATVFGT ACSLGLGALQ IGAGLSAANI IEDPSDWTIV GIVSVLTLAF IFSAISGVGK 
301:	GIQYLSNANM VLAALLAIFV FVVGPTVSIL NLLPGSIGNY LSNFFQMAGR TAMSADGTAG 
361:	EWLGSWTIFY WAWWISWSPF VGMFLARISR GRSIREFILG VLLVPAGVST VWFSIFGGTA 
421:	IVFEQNGESI WGDGAAEEQL FGLLHALPGG QIMGIIAMIL LGTFFITSAD SASTVMGTMS 
481:	QHGQLEANKW VTAAWGVATA AIGLTLLLSG GDNALSNLQN VTIVAATPFL FVVIGLMFAL 
541:	VKDLSNDVIY LEYREQQRFN ARLARERRVH NEHRKRELAA KRRRERKASG AGKRR