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2.A.36.1.1
Na+:H+ antiporter 1 (Nhe-1) (Regulated by Daxx (O35613)). An integral membrane protein that regulates intracellular pH and has a large N-terminal membrane domain of 12 transmembrane segments and an intracellular C-terminal regulatory domain (Reddy et al., 2008). The dimer catalyzes antiport with 2Na+/2H+ stoichiometry (Fuster et al., 2008). Nedd4-1 and β-arrestin-1 are key regulators of Na+/H+ exchanger 1 ubiquitylation, endocytosis and function (Simonin and Fuster et al., 2010). Important in heart disease and cancer. Structural studies have been performed using NMR (Lee et al., 2011).

Accession Number:P26431
Protein Name:NAH1 aka Nhe-1 aka SLC9A1 aka NHE1
Length:820
Molecular Weight:91647.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Na+, H+

Cross database links:

Genevestigator: P26431
eggNOG: roNOG04790
RefSeq: NP_036784.1   
Entrez Gene ID: 24782   
Pfam: PF00999   
KEGG: rno:24782   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0016021 C:integral to membrane
GO:0045121 C:membrane raft
GO:0015385 F:sodium:hydrogen antiporter activity
GO:0008134 F:transcription factor binding
GO:0030154 P:cell differentiation
GO:0016049 P:cell growth
GO:0045768 P:positive regulation of anti-apoptosis
GO:0007243 P:intracellular protein kinase cascade
GO:0006885 P:regulation of pH
GO:0042493 P:response to drug
GO:0014070 P:response to organic cyclic substance
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (4)

[1] “Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins.”  Orlowski J.et.al.   1577762
[2] “Topological analysis of NHE1, the ubiquitous Na+/H+ exchanger using chymotryptic cleavage.”  Shrode L.D.et.al.   9688597
[3] “Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS.”  Moser K.et.al.   16396499
[4] “Calcineurin B homologous protein 3 promotes the biosynthetic maturation, cell surface stability, and optimal transport of the Na+/H+ exchanger NHE1 isoform.”  Zaun H.C.et.al.   18321853

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MMLRWSGIWG LYPPRIFPSL LVVVALVGLL PVLRSHGLQL NPTASTIRGS EPPRERSIGD 
61:	VTTAPSEPLH HPDDRNLTNL YIEHGAKPVR KAFPVLDIDY LHVRTPFEIS LWILLACLMK 
121:	IGFHVIPTIS SIVPESCLLI VVGLLVGGLI KGVGETPPFL QSDVFFLFLL PPIILDAGYF 
181:	LPLRQFTENL GTILIFAVVG TLWNAFFLGG LLYAVCLVGG EQINNIGLLD TLLFGSIISA 
241:	VDPVAVLAVF EEIHINELLH ILVFGESLLN DAVTVVLYHL FEEFASYEYV GISDIFLGFL 
301:	SFFVVSLGGV FVGVVYGVIA AFTSRFTSHI RVIEPLFVFL YSYMAYLSAE LFHLSGIMAL 
361:	IASGVVMRPY VEANISHKSH TTIKYFLKMW SSVSETLIFI FLGVSTVAGS HQWNWTFVIS 
421:	TLLFCLIARV LGVLVLTWFI NKFRIVKLTP KDQFIIAYGG LRGAIAFSLG YLLDKKHFPM 
481:	CDLFLTAIIT VIFFTVFVQG MTIRPLVDLL AVKKKQETKR SINEEIHTQF LDHLLTGIED 
541:	ICGHYGHHHW KDKLNRFNKK YVKKCLIAGE RSKEPQLIAF YHKMEMKQAI ELVESGGMGK 
601:	IPSAVSTVSM QNIHPKSAAS ERILPALSKD KEEEIRKILR SNLQKTRQRL RSYNRHTLVA 
661:	DPYEEAWNQM LLRRQKARQL EQKITNYLTV PAHKLDSPTM SRARIGSDPL AYEPKADLPV 
721:	ITIDPASPQS PESVDLVNEE LKGKVLGLKR GPRTTPEEEE EDEDGVIMIR SKEPSSPGTD 
781:	DVFTPGPSDS PGSQRIQRCL SDPGPHPEPG EGEPFIPKGQ